| First Author | Chung SS | Year | 2004 |
| Journal | Transgenic Res | Volume | 13 |
| Issue | 3 | Pages | 289-94 |
| PubMed ID | 15359605 | Mgi Jnum | J:168137 |
| Mgi Id | MGI:4881913 | Doi | 10.1023/b:trag.0000034716.73957.f7 |
| Citation | Chung SS, et al. (2004) Primary spermatocyte-specific Cre recombinase activity in transgenic mice. Transgenic Res 13(3):289-94 |
| abstractText | We have evaluated the specificity of Cre recombinase activity in transgenic mice expressing Cre under the control of the synatonemal complex protein 1 (Sycp1) gene promoter. Sycp1Cre mice were crossed with the ROSA26 reporter line R26R, to monitor the male germ cell stage-specificity of Cre activity as well as to verify that Cre was not active previously during development of other tissues. X-gal staining detected Cre-mediated recombination only in testis. Detailed histological examination indicated that weak Cre-mediated recombination occurred as early as in zygotene spermatocytes at stage XI of the cycle of the seminiferous epithelium. Robust expression of X-gal was detected in early to mid-late spermatocytes at stages V-VIII. We conclude that this transgenic line is a powerful tool for deleting genes of interest specifically during male meiosis. |
