| First Author | Inselman AL | Year | 2010 |
| Journal | Genesis | Volume | 48 |
| Issue | 2 | Pages | 114-20 |
| PubMed ID | 20027617 | Mgi Jnum | J:156314 |
| Mgi Id | MGI:4420325 | Doi | 10.1002/dvg.20588 |
| Citation | Inselman AL, et al. (2010) Heat shock protein 2 promoter drives cre expression in spermatocytes of transgenic mice. Genesis 48(2):114-20 |
| abstractText | We generated transgenic mouse line C57BL/6-Tg(Hspa2-cre)1Eddy/J (Hspa2-cre), which expresses cre-recombinase under the control of a 907-bp fragment of the heat shock protein 2 (Hspa2) gene promoter. Transgene expression was determined using Gt(ROSA)26Sor(tm1Sor)/J (ROSA26) and Tg(CAG-Bgeo/GFP)21Lbe/J (Z/EG) reporter strains and RT-PCR and immunohistochemistry assays. Hspa2-cre expression mimicked the spermatogenic cell-specific expression of endogenous HSPA2 within the testis, being first observed in leptotene/zygotene spermatocytes. Expression of the transgene also was detected at restricted sites in the brain, as occurs for endogenous HSPA2. Although the results of mating the Hspa2-cre mice to mice with a floxed Cdc2a allele indicated that some expression of the transgene occurs during embryogenesis, the Hspa2-cre mice provide a valuable new tool for assessing the roles of genes during and after meiotic prophase in pachytene spermatocytes. genesis 00:1-7, 2010. Published 2009 Wiley-Liss, Inc. |
