| First Author | Miwa H | Year | 2015 |
| Journal | Genesis | Volume | 53 |
| Issue | 5 | Pages | 329-36 |
| PubMed ID | 25884589 | Mgi Jnum | J:221424 |
| Mgi Id | MGI:5639156 | Doi | 10.1002/dvg.22853 |
| Citation | Miwa H, et al. (2015) Generation and characterization of PDGFRalpha-GFPCreER(t2) knock-In mouse line. Genesis 53(5):329-36 |
| abstractText | Platelet-derived growth factor (PDGF) and its receptor play an important role in embryogenesis. PDGF receptor alpha (PDGFRalpha) is expressed specifically in the embryonic day 7.5 (E7.5) mesoderm and in the E9.5 neural crest among other tissues. PDGFRalpha-expressing cells and their descendants are involved in the formation of various tissues. To trace PDGFRalpha-expressing cells in vivo, we generated a knock-in mouse line that expressed a fusion protein of green fluorescent protein (GFP), Cre recombinase (Cre), and mutated estrogen receptor ligand-binding domain (ER(T2) ) under the control of the PDGFRalpha promoter. In these mice, Cre activity in PDGFRalpha-expressing cells could be induced by tamoxifen treatment. Taken together, our results suggest that the knock-in mouse line generated here could be useful for studying PDGFRalpha-expressing cells and their descendants in vivo at various stages of development. genesis 53:329-336, 2015. (c) 2015 Wiley Periodicals, Inc. |
