| First Author | Martin WD | Year | 1996 |
| Journal | Cell | Volume | 84 |
| Issue | 4 | Pages | 543-50 |
| PubMed ID | 8598041 | Mgi Jnum | J:31625 |
| Mgi Id | MGI:79111 | Doi | 10.1016/s0092-8674(00)81030-2 |
| Citation | Martin WD, et al. (1996) H2-M mutant mice are defective in the peptide loading of class II molecules, antigen presentation, and T cell repertoire selection. Cell 84(4):543-50 |
| abstractText | H2-M is a nonconventional major histocompatibility complex (MHC) class II molecule that has been implicated in the loading of peptides onto conventional class II molecules. We generated mice with a targeted mutation in the H2-Ma gene, which encodes a subunit for H2-M. Although the mutant mice express normal class II cell surface levels, these are structurally distinct from the compact SDS- resistant complexes expressed by wild-type cells and are predominantly bound by class II-associated invariant chain peptides (CLIPs). Cells from these animals are unable to present intact protein antigens to class II-restricted T cells and show reduced capacity to present exogenous peptides. Numbers of mature CD4(+) T lymphocytes in mutant mice are reduced 3- to 4-fold and exhibit altered reactivities. Overall, this phenotype establishes an important role for H2-M in regulating MHC class II function in vivo and supports the notion that self- peptides contribute to the specificity of T cell positive selection. |
