| First Author | Golovkina T | Year | 2001 |
| Journal | J Immunol | Volume | 166 |
| Issue | 4 | Pages | 2244-50 |
| PubMed ID | 11160278 | Mgi Jnum | J:126987 |
| Mgi Id | MGI:3762456 | Doi | 10.4049/jimmunol.166.4.2244 |
| Citation | Golovkina T, et al. (2001) Diverse repertoire of the MHC class II-peptide complexes is required for presentation of viral superantigens. J Immunol 166(4):2244-50 |
| abstractText | Among other features, peptides affect MHC class II molecules, causing changes in the binding of bacterial superantigens (b-Sag). Whether peptides can alter binding of viral superantigens (v-Sag) to MHC class II was not known. Here we addressed the question of whether mutations limiting the diversity of peptides bound by the MHC class II molecules influenced the presentation of v-Sag and, subsequently, the life cycle of the mouse mammary tumor virus (MMTV). T cells reactive to v-Sag were found in mice lacking DM molecules as well as in A(b)Ep-transgenic mice in which MHC class II binding grooves were predominantly occupied by an invariant chain fragment or Ealpha(52-68) peptide, respectively. APCs from the mutant mice failed to present v-Sag, as determined by the lack of Sag-specific T cell activation, Sag-induced T cell deletion, and by the aborted MMTV infection. In contrast, mice that express I-A(b) with a variety of bound peptides presented v-Sag and were susceptible to MMTV infection. Comparison of v-Sag and b-Sag presentation by the same mutant cells suggested that presentation of v-Sag had requirements similar to that for presentation of toxic shock syndrome toxin-1. Thus, MHC class II peptide repertoire is critical for recognition of v-Sag by the T cells and affects the outcome of infection with a retrovirus. |
