| First Author | Lam KP | Year | 1997 |
| Journal | Cell | Volume | 90 |
| Issue | 6 | Pages | 1073-83 |
| PubMed ID | 9323135 | Mgi Jnum | J:79299 |
| Mgi Id | MGI:2387749 | Doi | 10.1016/s0092-8674(00)80373-6 |
| Citation | Lam KP, et al. (1997) In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death. Cell 90(6):1073-83 |
| abstractText | Gene targeting experiments have demonstrated that the expression of immunoglobulin heavy chain in the pre-B cell receptor (pBCR) and of heavy and light chains in the B cell antigen receptor (BCR) marks checkpoints in early B cell development that the cells have to pass to survive. To investigate whether the persistence of mature B cells in the peripheral immune system also depends on BCR expression, we have generated a transgenic mouse in which the BCR can be inducibly ablated through V region gene deletion. Ablation leads to rapid death of mature B lymphocytes, which is preceded by down-regulation of MHC antigens and up-regulation of CD95 (Fas) and can be delayed by constitutive bcl-2 expression. |
