| First Author | Boulanger DS | Year | 2010 |
| Journal | J Immunol | Volume | 184 |
| Issue | 1 | Pages | 73-83 |
| PubMed ID | 19949070 | Mgi Jnum | J:159043 |
| Mgi Id | MGI:4441102 | Doi | 10.4049/jimmunol.0803489 |
| Citation | Boulanger DS, et al. (2010) Absence of tapasin alters immunodominance against a lymphocytic choriomeningitis virus polytope. J Immunol 184(1):73-83 |
| abstractText | Tapasin edits the peptide repertoire presented to CD8(+) T cells by favoring loading of slow off-rate peptides on MHC I molecules. To investigate the role of tapasin on T cell immunodominance we used poxvirus viral vectors expressing a polytope of lymphocytic choriomeningitis virus epitopes with different off-rates. In tapasin-deficient mice, responses to subdominant fast off-rate peptides were clearly favored. This alteration of the CD8(+) T cell hierarchy was a consequence of tapasin editing and not a consequence of the alteration of the T cell repertoire in tapasin-deficient mice, because bone marrow chimeric mice (wild-type recipients reconstituted with tapasin knockout bone marrow) showed the same hierarchy as the tapasin knockout mice. Tapasin editing is therefore a contributing factor to the phenomenon of immunodominance. Although tapasin knockout cells have low MHC I surface expression, Ag presentation was efficient and resulted in strong T cell responses involving T cells with increased functional avidity. Therefore, in this model, tapasin-deficient mice do not have a reduced but rather have an altered immune response. |
