| First Author | Pang Y | Year | 2024 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 65 |
| Issue | 5 | Pages | 36 |
| PubMed ID | 38776115 | Mgi Jnum | J:354518 |
| Mgi Id | MGI:7644638 | Doi | 10.1167/iovs.65.5.36 |
| Citation | Pang Y, et al. (2024) CD38 Deficiency Protects Mouse Retinal Ganglion Cells Through Activating the NAD+/Sirt1 Pathway in Ischemia-Reperfusion and Optic Nerve Crush Models. Invest Ophthalmol Vis Sci 65(5):36 |
| abstractText | PURPOSE: The purpose of this study was to investigate the protective effect of CD38 deletion on retinal ganglion cells (RGCs) in a mouse retinal ischemia/reperfusion (I/R) model and an optic nerve crush (ONC) model, and to elucidate the underlying molecular mechanisms. METHODS: Retinal I/R and ONC models were constructed in mice. PCR was used to identify the deletion of CD38 gene in mice, hematoxylin and eosin (H&E) staining was used to evaluate the changes in retinal morphology, and electroretinogram (ERG) was used to evaluate the changes in retinal function. The survival of RGCs and activation of retinal macroglia were evaluated by immunofluorescence staining. The expression of Sirt1, CD38, Ac-p65, Ac-p53, TNF-alpha, IL-1beta, and Caspase3 proteins in the retina was further evaluated by protein imprinting. RESULTS: In retinal I/R and ONC models, CD38 deficiency reduced the loss of RGCs and activation of macroglia and protected the retinal function. CD38 deficiency increased the concentration of NAD+, reduced the degree of acetylation of NF-kappaB p65 and p53, and reduced expression of the downstream inflammatory cytokines TNFalpha, IL-1beta, and apoptotic protein Caspase3 in the retina in the ONC model. Intraperitoneal injection of the Sirt1 inhibitor EX-527 partially counteracted the effects of CD38 deficiency, suggesting that CD38 deficiency acts at least in part through the NAD+/Sirt1 pathway. CONCLUSIONS: CD38 plays an important role in the pathogenesis of retinal I/R and ONC injury. CD38 deletion protects RGCs by attenuating inflammatory responses and apoptosis through the NAD+/Sirt1 pathway. |
