| First Author | Zaidan N | Year | 2022 |
| Journal | Stem Cell Reports | Volume | 17 |
| Issue | 8 | Pages | 1788-1798 |
| PubMed ID | 35905741 | Mgi Jnum | J:327428 |
| Mgi Id | MGI:7332442 | Doi | 10.1016/j.stemcr.2022.06.008 |
| Citation | Zaidan N, et al. (2022) Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation. Stem Cell Reports 17(8):1788-1798 |
| abstractText | To generate sufficient numbers of transplantable hematopoietic stem cells (HSCs) in vitro, a detailed understanding of how this process takes place in vivo is essential. The endothelial-to-hematopoietic transition (EHT), which culminates in the production of the first HSCs, is a highly complex process during which key regulators are switched on and off at precise moments, and that is embedded into a myriad of microenvironmental signals from surrounding cells and tissues. We have previously demonstrated an HSC-supportive function for GATA3 within the sympathetic nervous system and the sub-aortic mesenchyme, but show here that it also plays a cell-intrinsic role during the EHT. It is expressed in hemogenic endothelial cells and early HSC precursors, where its expression correlates with a more quiescent state. Importantly, endothelial-specific deletion of Gata3 shows that it is functionally required for these cells to mature into HSCs, placing GATA3 at the core of the EHT regulatory network. |
