| First Author | Rosas LE | Year | 2005 |
| Journal | Int Immunol | Volume | 17 |
| Issue | 10 | Pages | 1347-57 |
| PubMed ID | 16141242 | Mgi Jnum | J:101701 |
| Mgi Id | MGI:3604836 | Doi | 10.1093/intimm/dxh313 |
| Citation | Rosas LE, et al. (2005) Genetic background influences immune responses and disease outcome of cutaneous L. mexicana infection in mice. Int Immunol 17(10):1347-57 |
| abstractText | The experimental model of high-dose Leishmania mexicana infection is used frequently to study molecular mechanisms regulating T(h)2 response since most inbred mice regardless of their genetic background display T(h)2 cytokine-dependent susceptibility to L. mexicana unlike Leishmania major. Here, we analyzed the course of L. mexicana infection in BALB/c, C57BL/6 and CBA/J mouse strains using low-dose ear infection model that mimics natural transmission. Although all three strains were equally susceptible to high-dose back rump L. mexicana infection, they displayed marked differences in their ability to control parasite growth after low-dose ear infection. Leishmania mexicana-infected BALB/c mice produced high levels of T(h)2-associated cytokines and developed non-healing lesions full of parasites, whereas CBA/J mice preferentially produced T(h)1-associated IFN-gamma but low levels of IL-4, and developed small self-resolving lesions. Both BALB/c and C57BL/6 mice produced comparable amounts of IFN-gamma following L. mexicana infection, but later produced less T(h)2-associated cytokines, and exhibited an 'intermediate' susceptibility phenotype characterized by lesion sizes that were significantly smaller than BALB/c mice but larger than CBA/J mice. Interestingly, all three strains also showed marked differences in trafficking of macrophages, CD4+ T cells and CD8+ T cells into their lesions. Finally, we analyzed the course of low-dose L. mexicana infection in signal transducers and activators of transcription (STAT) 6-/- and STAT6+/+ BALB/c mice. We found that STAT6-/- mice mount a T(h)1 response, produce high levels of IL-12 and IFN-gamma and develop smaller lesions containing fewer parasites as compared with STAT6+/+ mice. Our findings demonstrate that genetic background plays a critical role in determining susceptibility of inbred mice to low-dose L. mexicana infection. Furthermore, together with our previous findings, they show that STAT6-mediated signaling is involved in mediating susceptibility to L. mexicana following both high-dose back rump and low-dose ear dermis infection. |
