| First Author | Solymar DC | Year | 2002 |
| Journal | Immunity | Volume | 17 |
| Issue | 1 | Pages | 41-50 |
| PubMed ID | 12150890 | Mgi Jnum | J:78089 |
| Mgi Id | MGI:2183324 | Doi | 10.1016/s1074-7613(02)00334-5 |
| Citation | Solymar DC, et al. (2002) A 3' enhancer in the IL-4 gene regulates cytokine production by Th2 cells and mast cells. Immunity 17(1):41-50 |
| abstractText | Differentiation of naive T cells into mature Th2 cells is associated with the appearance of a complex pattern of DNase I hypersensitive (DH) sites within the IL-4/IL-13 cytokine gene cluster. We show here that targeted deletion of an inducible DH site, V(A), and the adjacent conserved DH site V (CNS-2) selectively compromises IL-4 gene transcription by differentiated Th2 cells and mast cells. In mast cells, the deletion abrogates IL-4 mRNA induction, an effect mimicked by deletion of the transcription factor NFAT1 (NFATc2), which binds DH site V(A). In T cells, the deletion impairs a process of response maturation, defined by progressive increases in IL-4 levels as Th2 differentiation proceeds. These results identify an essential enhancer which regulates IL-4 gene expression in two important cell lineages in vivo. |
