| First Author | Liu AJ | Year | 2012 |
| Journal | CNS Neurosci Ther | Volume | 18 |
| Issue | 11 | Pages | 918-26 |
| PubMed ID | 23106973 | Mgi Jnum | J:309954 |
| Mgi Id | MGI:6760032 | Doi | 10.1111/cns.12011 |
| Citation | Liu AJ, et al. (2012) Involvement of acetylcholine-alpha7nAChR in the protective effects of arterial baroreflex against ischemic stroke. CNS Neurosci Ther 18(11):918-26 |
| abstractText | AIMS: Decreased baroreflex sensitivity is associated with poor outcome in many cardiovascular diseases including stroke, but the molecular mechanism underlying this relationship is unclear. This work was designed to test the hypothesis that acetylcholine (ACh) and alpha7 nicotinic ACh receptor (alpha7nAChR) mediate the protection of arterial baroreflex against stroke. METHODS: Sinoaortic denervation (SAD) was used to impair the function of arterial baroreflex, and anticholinesterase agents were used to activate the cholinergic system and increase endogenous ACh. Middle cerebral artery occlusion (MCAO) was performed in the alpha7nAChR knockout (KO) mice and Sprague-Dawley rats. RESULTS: We found decreased expression of vesicular ACh transporter (VAChT) and alpha7nAChR in rat brain after SAD. In rats subjected to MCAO, neostigmine significantly reduced the infarct size. The protective effects of neostigmine were abolished by selective nAChR antagonist vecuronium but not by mAChR antagonist anisodamine. In addition, the effect of neostigmine disappeared in alpha7nAChR KO mice. In cultured neurons, ACh inhibited cell death induced by H(2) O(2) . In cultured microglial cells, ACh decreased the release of proinflammatory cytokines induced by lipopolysaccharide. These in vitro effects were blocked by selective alpha7nAChR antagonists. CONCLUSION: Taken together, these findings indicate that the ACh-alpha7nAChR involved in the protective effects of arterial baroreflex against ischemic stroke. |
