| First Author | Murshed M | Year | 2000 |
| Journal | Mol Cell Biol | Volume | 20 |
| Issue | 18 | Pages | 7007-12 |
| PubMed ID | 10958695 | Mgi Jnum | J:64030 |
| Mgi Id | MGI:1888616 | Doi | 10.1128/mcb.20.18.7007-7012.2000 |
| Citation | Murshed M, et al. (2000) The absence of nidogen 1 does not affect murine basement membrane formation. Mol Cell Biol 20(18):7007-12 |
| abstractText | Nidogen 1 is a highly conserved protein in mammals, Drosophila melanogaster, Caenorhabditis elegans, and ascidians and is found in all basement membranes. It has been proposed that nidogen 1 connects the laminin and collagen IV networks, so stabilizing the basement membrane, and integrates other proteins, including perlecan, into the basement membrane. To define the role of nidogen 1 in basement membranes in vivo, we produced a null mutation of the NID-1 gene in embryonic stem cells and used these to derive mouse lines. Homozygous animals produce neither nidogen 1 mRNA nor protein. Surprisingly, they show no overt abnormalities and are fertile, their basement membrane structures appearing normal. Nidogen 2 staining is increased in certain basement membranes, where it is normally only found in scant amounts. This occurs by either redistribution from other extracellular matrices or unmasking of nidogen 2 epitopes, as its production does not appear to be upregulated. The results show that nidogen 1 is not required for basement membrane formation or maintenance. |
