| First Author | Balzano M | Year | 2019 |
| Journal | Cell Rep | Volume | 26 |
| Issue | 12 | Pages | 3257-3271.e8 |
| PubMed ID | 30893599 | Mgi Jnum | J:283550 |
| Mgi Id | MGI:6380777 | Doi | 10.1016/j.celrep.2019.02.065 |
| Citation | Balzano M, et al. (2019) Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep 26(12):3257-3271.e8 |
| abstractText | In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1(-/-) mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7(+) stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages. |
