| First Author | Pierce AJ | Year | 2001 |
| Journal | Genes Dev | Volume | 15 |
| Issue | 24 | Pages | 3237-42 |
| PubMed ID | 11751629 | Mgi Jnum | J:73443 |
| Mgi Id | MGI:2155497 | Doi | 10.1101/gad.946401 |
| Citation | Pierce AJ, et al. (2001) Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells. Genes Dev 15(24):3237-42 |
| abstractText | Chromosomal double-strand breaks (DSBs) in mammalian cells are repaired by either homology-directed repair (HDR), using a homologous sequence as a repair template, or nonhomologous end-joining (NHEJ), which often involves sequence alterations at the DSB site. To characterize the interrelationship of these two pathways, we analyzed HDR of a DSB in cells deficient for NHEJ components. We find that the HDR frequency is enhanced in Ku70(-/-), XRCC4(-/-), and DNA-PKcs(-/-) cells, with the increase being particularly striking in Ku70(-/-) cells. Neither sister-chromatid exchange nor gene-targeting frequencies show a dependence on these NHEJ proteins. A Ku-modulated two-ended versus one-ended chromosome break model is presented to explain these results. |
