| First Author | Cibrian D | Year | 2020 |
| Journal | J Allergy Clin Immunol | Volume | 145 |
| Issue | 1 | Pages | 199-214.e11 |
| PubMed ID | 31605740 | Mgi Jnum | J:293218 |
| Mgi Id | MGI:6452246 | Doi | 10.1016/j.jaci.2019.09.025 |
| Citation | Cibrian D, et al. (2020) Targeting L-type amino acid transporter 1 in innate and adaptive T cells efficiently controls skin inflammation. J Allergy Clin Immunol 145(1):199-214.e11 |
| abstractText | BACKGROUND: Psoriasis is a frequent inflammatory skin disease that is mainly mediated by IL-23, IL-1beta, and IL-17 cytokines. Although psoriasis is a hyperproliferative skin disorder, the possible role of amino acid transporters has remained unexplored. OBJECTIVE: We sought to investigate the role of the essential amino acid transporter L-type amino acid transporter (LAT) 1 (SLC7A5) in psoriasis. METHODS: LAT1 floxed mice were crossed to Cre-expressing mouse strains under the control of keratin 5, CD4, and retinoic acid receptor-related orphan receptor gamma. We produced models of skin inflammation induced by imiquimod (IMQ) and IL-23 and tested the effect of inhibiting LAT1 (JPH203) and mammalian target of rapamycin (mTOR [rapamycin]). RESULTS: LAT1 expression is increased in keratinocytes and skin-infiltrating lymphocytes of psoriatic lesions in human subjects and mice. LAT1 deletion in keratinocytes does not dampen the inflammatory response or their proliferation, which could be maintained by increased expression of the alternative amino acid transporters LAT2 and LAT3. Specific deletion of LAT1 in gammadelta and CD4 T cells controls the inflammatory response induced by IMQ. LAT1 deletion or inhibition blocks expansion of IL-17-secreting gamma4(+)delta4(+) and CD4 T cells and dampens the release of IL-1beta, IL-17, and IL-22 in the IMQ-induced model. Moreover, inhibition of LAT1 blocks expansion of human gammadelta T cells and IL-17 secretion by human CD4 T cells. IL-23 and IL-1beta stimulation upregulates LAT1 expression and induces mTOR activation in IL-17(+) gammadelta and TH17 cells. Deletion or inhibition of LAT1 efficiently controls IL-23- and IL-1beta-induced phosphatidylinositol 3-kinase/AKT/mTOR activation independent of T-cell receptor signaling. CONCLUSION: Targeting LAT1-mediated amino acid uptake is a potentially useful immunosuppressive strategy to control skin inflammation mediated by the IL-23/IL-1beta/IL-17 axis. |
