| First Author | Moore R | Year | 2014 |
| Journal | Mol Cell Biol | Volume | 34 |
| Issue | 3 | Pages | 560-72 |
| PubMed ID | 24277939 | Mgi Jnum | J:315320 |
| Mgi Id | MGI:6830085 | Doi | 10.1128/MCB.00937-13 |
| Citation | Moore R, et al. (2014) The primitive endoderm segregates from the epiblast in beta1 integrin-deficient early mouse embryos. Mol Cell Biol 34(3):560-72 |
| abstractText | We analyzed the mechanism of developmental failure in implanted beta1 integrin-null blastocysts and found that primitive endoderm cells are present but segregate away from, instead of forming an epithelial layer covering, the inner cell mass. This cell segregation phenotype was also reproduced in beta1 integrin-null embryoid bodies, in which primitive endoderm cells segregated and appeared as miniature aggregates detached from the core spheroids, and a primitive endoderm layer failed to form on the surface. Restricted beta1 integrin gene deletion in embryos using Ttr-Cre or Sox2-Cre indicated that the loss of integrin function in the cells of the inner core rather than the outer layer is responsible for the failure to form a primitive endoderm layer. We conclude that beta1 integrin is essential for the attachment of the primitive endoderm layer to the epiblast during the formation of a basement membrane, a process concurrent with the transition from cadherin- to integrin-mediated cell adhesion. |
