| First Author | Schober M | Year | 2011 |
| Journal | Proc Natl Acad Sci U S A | Volume | 108 |
| Issue | 26 | Pages | 10544-9 |
| PubMed ID | 21670270 | Mgi Jnum | J:173553 |
| Mgi Id | MGI:5014445 | Doi | 10.1073/pnas.1107807108 |
| Citation | Schober M, et al. (2011) Tumor-initiating stem cells of squamous cell carcinomas and their control by TGF-{beta} and integrin/focal adhesion kinase (FAK) signaling. Proc Natl Acad Sci U S A 108(26):10544-9 |
| abstractText | Cancer stem cells (CSCs) sustain tumor growth through their ability to self-renew and to generate differentiated progeny. These functions endow CSCs with the potential to initiate secondary tumors bearing characteristics similar to those of the parent. Recently the hair follicle stem cell marker CD34 was used to purify a CSC-like cell population from early skin tumors arising from treatment with 7,12-dimethylbenz[alpha]anthracene/12-o-tetradecanoylphorbol-13-acetate, which typically generates benign papillomas that occasionally progress to squamous cell carcinomas (SCCs). In the present study, we identify and characterize CSCs purified from malignant SCCs. We show that SCCs contain two highly tumorigenic CSC populations that differ in CD34 levels but are enriched for integrins and coexist at the SCC-stroma interface. Intriguingly, whether CD34(lo) or CD34(hi), alpha6(hi)beta1(hi) populations can initiate secondary tumors by serial limit-dilution transplantation assays, but alpha6(lo)beta1(lo) populations cannot. Moreover, secondary tumors generated from a single CSC of either subtype contain both CD34(lo) and CD34(hi) alpha6(hi)beta1(hi)CSCs, indicating their nonhierarchical organization. Genomic profiling and hierarchical cluster analysis show that these two CSC subtypes share a molecular signature distinct from either the CD34(-) epidermal or the CD34(hi) hair follicle stem cell signature. Although closely related, alpha6(hi)beta1(hi)CD34(lo) and alpha6(hi)beta1(hi)CD34(hi) CSCs differ in cell-cycle gene expression and proliferation characteristics. Indeed, proliferation and expansion of alpha6(hi)beta1(hi)CD34(hi) CSCs is sensitive to whether they can initiate a TGF-beta receptor II-mediated response to counterbalance elevated focal adhesion kinase-mediated integrin signaling within the tumor. Overall, the coexistence and interconvertibility of CSCs with differing sensitivities to their microenvironment pose challenges and opportunities for SCC cancer therapies. |
