| First Author | Cui W | Year | 2021 |
| Journal | Curr Biol | Volume | 31 |
| Issue | 15 | Pages | 3330-3342.e7 |
| PubMed ID | 34143959 | Mgi Jnum | J:321279 |
| Mgi Id | MGI:6741166 | Doi | 10.1016/j.cub.2021.05.045 |
| Citation | Cui W, et al. (2021) In trans neuregulin3-Caspr3 interaction controls DA axonal bassoon cluster development. Curr Biol 31(15):3330-3342.e7 |
| abstractText | Dopamine (DA) transmission is critical to motivation, movement, and emotion. Unlike glutamatergic and GABAergic synapses, the development of DA synapses is less understood. We show that bassoon (BSN) clusters along DA axons in the core of nucleus accumbens (NAcc) were increased in neonatal stages and reduced afterward, suggesting DA synapse elimination. Remarkably, DA neuron-specific ablating neuregulin 3 (NRG3), a protein whose levels correlate with BSN clusters, increased the clusters and impaired DA release and behaviors related to DA transmission. An unbiased screen of transmembrane proteins with the extracellular domain (ECD) of NRG3 identified Caspr3 (contactin associate-like protein 3) as a binding partner. Caspr3 was enriched in striatal medium spiny neurons (MSNs). NRG3 and Caspr3 interact in trans, which was blocked by Caspr3-ECD. Caspr3 null mice displayed phenotypes similar to those in DAT-Nrg3(f/f) mice in DA axonal BSN clusters and DA transmission. Finally, in vivo disruption of the NRG3-Caspr3 interaction increased BSN clusters. Together, these results demonstrate that DA synapse development is controlled by trans interaction between NRG3 in DA neurons and Caspr3 in MSNs, identifying a novel pair of cell adhesion molecules for brain circuit wiring. |
