| First Author | Manoharan Valerio M | Year | 2023 |
| Journal | Front Immunol | Volume | 14 |
| Pages | 1250316 | PubMed ID | 38022509 |
| Mgi Jnum | J:347859 | Mgi Id | MGI:7563043 |
| Doi | 10.3389/fimmu.2023.1250316 | Citation | Manoharan Valerio M, et al. (2023) The promiscuous development of an unconventional Qa1b-restricted T cell population. Front Immunol 14:1250316 |
| abstractText | MHC-E restricted CD8 T cells show promise in vaccine settings, but their development and specificity remain poorly understood. Here we focus on a CD8 T cell population reactive to a self-peptide (FL9) bound to mouse MHC-E (Qa-1(b)) that is presented in response to loss of the MHC I processing enzyme ERAAP, termed QFL T cells. We find that mature QFL thymocytes are predominantly CD8alphabeta+CD4-, show signs of agonist selection, and give rise to both CD8alphaalpha and CD8alphabeta intraepithelial lymphocytes (IEL), as well as memory phenotype CD8alphabeta T cells. QFL T cells require the MHC I subunit beta-2 microglobulin (beta2m), but do not require Qa1(b) or classical MHC I for positive selection. However, QFL thymocytes do require Qa1(b) for agonist selection and full functionality. Our data highlight the relaxed requirements for positive selection of an MHC-E restricted T cell population and suggest a CD8alphabeta+CD4- pathway for development of CD8alphaalpha IELs. |
