| First Author | Mansour SL | Year | 1988 |
| Journal | Nature | Volume | 336 |
| Issue | 6197 | Pages | 348-52 |
| PubMed ID | 3194019 | Mgi Jnum | J:28595 |
| Mgi Id | MGI:76117 | Doi | 10.1038/336348a0 |
| Citation | Mansour SL, et al. (1988) Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 336(6197):348-52 |
| abstractText | Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene. |
