| First Author | Perrin BJ | Year | 2010 |
| Journal | PLoS Genet | Volume | 6 |
| Issue | 10 | Pages | e1001158 |
| PubMed ID | 20976199 | Mgi Jnum | J:167543 |
| Mgi Id | MGI:4868526 | Doi | 10.1371/journal.pgen.1001158 |
| Citation | Perrin BJ, et al. (2010) beta-actin and gamma-actin are each dispensable for auditory hair cell development but required for Stereocilia maintenance. PLoS Genet 6(10):e1001158 |
| abstractText | Hair cell stereocilia structure depends on actin filaments composed of cytoplasmic beta-actin and gamma-actin isoforms. Mutations in either gene can lead to progressive hearing loss in humans. Since beta-actin and gamma-actin isoforms are 99% identical at the protein level, it is unclear whether each isoform has distinct cellular roles. Here, we compared the functions of beta-actin and gamma-actin in stereocilia formation and maintenance by generating mice conditionally knocked out for Actb or Actg1 in hair cells. We found that, although cytoplasmic actin is necessary, neither beta-actin nor gamma-actin is required for normal stereocilia development or auditory function in young animals. However, aging mice with beta-actin- or gamma-actin-deficient hair cells develop different patterns of progressive hearing loss and distinct pathogenic changes in stereocilia morphology, despite colocalization of the actin isoforms. These results demonstrate overlapping developmental roles but unique post-developmental functions for beta-actin and gamma-actin in maintaining hair cell stereocilia. |
