| First Author | Jiang W | Year | 2019 |
| Journal | J Immunol | Volume | 203 |
| Issue | 1 | Pages | 178-187 |
| PubMed ID | 31101667 | Mgi Jnum | J:276977 |
| Mgi Id | MGI:6315825 | Doi | 10.4049/jimmunol.1801657 |
| Citation | Jiang W, et al. (2019) Phosphorylation at S2053 in Murine (S2056 in Human) DNA-PKcs Is Dispensable for Lymphocyte Development and Class Switch Recombination. J Immunol 203(1):178-187 |
| abstractText | The classical nonhomologous end-joining (cNHEJ) pathway is a major DNA double-strand break repair pathway in mammalian cells and is required for lymphocyte development and maturation. The DNA-dependent protein kinase (DNA-PK) is a cNHEJ factor that encompasses the Ku70-Ku80 (KU) heterodimer and the large DNA-PK catalytic subunit (DNA-PKcs). In mouse models, loss of DNA-PKcs (DNA-PKcs(-/-) ) abrogates end processing (e.g., hairpin opening), but not end-ligation, whereas expression of the kinase-dead DNA-PKcs protein (DNA-PKcs(KD/KD) ) abrogates end-ligation, suggesting a kinase-dependent structural function of DNA-PKcs during cNHEJ. Lymphocyte development is abolished in DNA-PKcs(-/-) and DNA-PKcs(KD/KD) mice because of the requirement for both hairpin opening and end-ligation during V(D)J recombination. DNA-PKcs itself is the best-characterized substrate of DNA-PK. The S2056 cluster is the best-characterized autophosphorylation site in human DNA-PKcs. In this study, we show that radiation can induce phosphorylation of murine DNA-PKcs at the corresponding S2053. We also generated knockin mouse models with alanine- (DNA-PKcs(PQR)) or phospho-mimetic aspartate (DNA-PKcs(SD)) substitutions at the S2053 cluster. Despite moderate radiation sensitivity in the DNA-PKcs(PQR/PQR) fibroblasts and lymphocytes, both DNA-PKcs(PQR/PQR) and DNA-PKcs(SD/SD) mice retained normal kinase activity and underwent efficient V(D)J recombination and class switch recombination, indicating that phosphorylation at the S2053 cluster of murine DNA-PKcs (corresponding to S2056 of human DNA-PKcs), although important for radiation resistance, is dispensable for the end-ligation and hairpin-opening function of DNA-PK essential for lymphocyte development. |
