| First Author | Schaft J | Year | 2001 |
| Journal | Genesis | Volume | 31 |
| Issue | 1 | Pages | 6-10 |
| PubMed ID | 11668672 | Mgi Jnum | J:129691 |
| Mgi Id | MGI:3769982 | Doi | 10.1002/gene.1076 |
| Citation | Schaft J, et al. (2001) Efficient FLP recombination in mouse ES cells and oocytes. Genesis 31(1):6-10 |
| abstractText | We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice. |
