| Primary Identifier | MGI:1932084 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Spp1 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Insertion of a neomycin resistance cassette into exon 6 immediately 5' of the RGD sequence disrupted the Spp1 gene. Northern analysis of homozygous mutant kidney detected low levels of a high molecular weight RNA species, but no wild-type or truncated Spp1 transcript. Immunoblot analysis detected low levels of a truncated form of the protein in bone but not in other tissues, fluids, or cells of homozygous mutant mice. |
