| Primary Identifier | MGI:2135614 | Allele Type | Spontaneous |
| Attribute String | Null/knockout | Gene | Poc1a |
| Inheritance Mode | Recessive | Strain of Origin | DBA.B6-A<hvy>/a x DBA/2J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 8 is disrupted by the insertion of a nearly full-length transcript (in reverse orientation) of centromere protein W (Cenpw) gene, including a 61 nucleotide poly-A tail that is 24 bp downstream from the common polyadenylation sequence AUUAAA. The total size of the insertion is 495 bp, and it includes a 5 untranslated region (UTR) lacking only seven bp relative to the published transcription start site, all three exons of Cenpw, and a 3UTR. Sequencing of the mutant RT-PCR product revealed that exon 8 is skipped precisely, preserving the reading frame of the transcript. Western blots were carried out to detect the expressed protein in tibiae. A single band of similar intensity was detected in wild type and mutant tibia, suggesting that a mutant protein is expressed. |
