| First Author | Ramon S | Year | 2012 |
| Journal | J Immunol | Volume | 189 |
| Issue | 10 | Pages | 4740-7 |
| PubMed ID | 23041568 | Mgi Jnum | J:190591 |
| Mgi Id | MGI:5449278 | Doi | 10.4049/jimmunol.1200956 |
| Citation | Ramon S, et al. (2012) Peroxisome proliferator-activated receptor gamma B cell-specific-deficient mice have an impaired antibody response. J Immunol 189(10):4740-7 |
| abstractText | Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily. PPARgamma, a ligand-activated transcription factor, has important anti-inflammatory and antiproliferative functions, and it has been associated with diseases including diabetes, scarring, and atherosclerosis, among others. PPARgamma is expressed in most bone marrow-derived cells and influences their function. PPARgamma ligands can stimulate human B cell differentiation and promote Ab production. A knowledge gap is that the role of PPARgamma in B cells under physiological conditions is not known. We developed a new B cell-specific PPARgamma (B-PPARgamma) knockout mouse and explored the role of PPARgamma during both the primary and secondary immune response. In this article, we show that PPARgamma deficiency in B cells decreases germinal center B cells and plasma cell development, as well as the levels of circulating Ag-specific Abs during a primary challenge. Inability to generate germinal center B cells and plasma cells is correlated to decreased MHC class II expression and decreased Bcl-6 and Blimp-1 levels. Furthermore, B-PPARgamma-deficient mice have an impaired memory response, characterized by low titers of Ag-specific Abs and low numbers of Ag-experienced, Ab-secreting cells. However, B-PPARgamma-deficient mice have no differences in B cell population distribution within primary or secondary lymphoid organs during development. This is the first report, to our knowledge, to show that, under physiological conditions, PPARgamma expression in B cells is required for an efficient B cell-mediated immune response as it regulates B cell differentiation and Ab production. |
