| First Author | Ueki H | Year | 2020 |
| Journal | Nat Protoc | Volume | 15 |
| Issue | 3 | Pages | 1041-1065 |
| PubMed ID | 31996843 | Mgi Jnum | J:360995 |
| Mgi Id | MGI:7855082 | Doi | 10.1038/s41596-019-0275-y |
| Citation | Ueki H, et al. (2020) Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS. Nat Protoc 15(3):1041-1065 |
| abstractText | In vivo two-photon imaging is a valuable technique for studies of viral pathogenesis and host responses to infection in vivo. In this protocol, we describe a methodology for analyzing influenza virus-infected lung in vivo by two-photon imaging microscopy. We describe the surgical procedure, how to stabilize the lung, and an approach to analyzing the data. Further, we provide a database of fluorescent dyes, antibodies, and reporter mouse lines that can be used in combination with a reporter influenza virus (Color-flu) for multicolor analysis. Setup of this model typically takes ~30 min and enables the observation of influenza virus-infected lungs for >4 h during the acute phase of the inflammation and at least 1 h in the lethal phase. This imaging system, which we termed two-photon IMPRESS (imaging pathophysiology research system), is broadly applicable to analyses of other respiratory pathogens and reveals disease progression at the cellular level in vivo. |
