| First Author | Daly J | Year | 2012 |
| Journal | J Immunol | Volume | 188 |
| Issue | 11 | Pages | 5528-37 |
| PubMed ID | 22547703 | Mgi Jnum | J:188723 |
| Mgi Id | MGI:5441663 | Doi | 10.4049/jimmunol.1102629 |
| Citation | Daly J, et al. (2012) Altered Ig hypermutation pattern and frequency in complementary mouse models of DNA polymerase zeta activity. J Immunol 188(11):5528-37 |
| abstractText | To test the hypothesis that DNA polymerase zeta participates in Ig hypermutation, we generated two mouse models of Pol zeta function: a B cell-specific conditional knockout and a knock-in strain with a Pol zeta mutagenesis-enhancing mutation. Pol zeta-deficient B cells had a reduction in mutation frequency at Ig loci in the spleen and in Peyer's patches, whereas knock-in mice with a mutagenic Pol zeta displayed a marked increase in mutation frequency in Peyer's patches, revealing a pattern that was similar to mutations in yeast strains with a homologous mutation in the gene encoding the catalytic subunit of Pol zeta. Combined, these data are best explained by a direct role for DNA polymerase zeta in Ig hypermutation. |
