| First Author | Coffey F | Year | 2010 |
| Journal | J Immunol | Volume | 184 |
| Issue | 9 | Pages | 4871-9 |
| PubMed ID | 20335528 | Mgi Jnum | J:160481 |
| Mgi Id | MGI:4454510 | Doi | 10.4049/jimmunol.0903506 |
| Citation | Coffey F, et al. (2010) Expression of cellular FLIP by B cells is required for their participation in an immune response. J Immunol 184(9):4871-9 |
| abstractText | High levels of the Fas-signaling antagonist cellular FLIP (cFLIP) in germinal center (GC) B cells suggests an important role for this factor during this stage of the T cell-dependent B cell immune response. To test this idea, we used mice with B cell-specific deletion of a floxed cFLIP allele. Although deletion of cFLIP did not alter their primary development, participation of cFLIP-deficient B cells in the immune response was severely perturbed. Using previously characterized IgH locus-targeted BCR transgenic mice, we showed that adoptively transferred cFLIP-deficient follicular B cells do not effectively participate in the GC response in wild-type hosts. However, this failure was accompanied by severe defects in the initial activation and proliferation of these B cells in vivo. In addition, immunization of mice with B cell-specific cFLIP deletion resulted in selective recruitment into GCs and Ab-forming cell responses of B cells that had not deleted the floxed cFLIP allele. Together, these findings demonstrate that expression of cFLIP is a prerequisite for participation of B cells in all stages of Ag-driven immune responses. |
