| First Author | Mouat IC | Year | 2022 |
| Journal | Sci Rep | Volume | 12 |
| Issue | 1 | Pages | 21189 |
| PubMed ID | 36477199 | Mgi Jnum | J:351052 |
| Mgi Id | MGI:7410572 | Doi | 10.1038/s41598-022-25543-1 |
| Citation | Mouat IC, et al. (2022) Age-associated B cells are long-lasting effectors that impede latent gammaHV68 reactivation. Sci Rep 12(1):21189 |
| abstractText | Age-associated B cells (ABCs; CD19(+)CD11c(+)T-bet(+)) are a unique population that are increased in an array of viral infections, though their role during latent infection is largely unexplored. Here, we use murine gammaherpesvirus 68 (gammaHV68) to demonstrate that ABCs remain elevated long-term during latent infection and express IFNgamma and TNF. Using a recombinant gammaHV68 that is cleared following acute infection, we show that ABCs persist in the absence of latent virus, though their expression of IFNgamma and TNF is decreased. With a fluorescent reporter gene-expressing gammaHV68 we demonstrate that ABCs are infected with gammaHV68 at similar rates to other previously activated B cells. We find that mice without ABCs display defects in anti-viral IgG2a/c antibodies and are more susceptible to reactivation of gammaHV68 following virus challenges that typically do not break latency. Together, these results indicate that ABCs are a persistent effector subset during latent viral infection that impedes gammaHV68 reactivation. |
