| First Author | Irani BG | Year | 2010 |
| Journal | Neuroscience | Volume | 170 |
| Issue | 4 | Pages | 1065-79 |
| PubMed ID | 20691763 | Mgi Jnum | J:166797 |
| Mgi Id | MGI:4849616 | Doi | 10.1016/j.neuroscience.2010.07.064 |
| Citation | Irani BG, et al. (2010) Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus. Neuroscience 170(4):1065-79 |
| abstractText | PKC-theta (PKC-theta), a member of the novel protein kinase C family (nPKC), regulates a wide variety of functions in the periphery. However, its presence and role in the CNS has remained largely unknown. Recently, we demonstrated the presence of PKC-theta in the arcuate hypothalamic nucleus (ARC) and knockdown of PKC-theta from the ARC protected mice from developing diet-induced obesity. Another isoform of the nPKC group, PKC-delta (PKC-delta), is expressed in several non-hypothalamic brain sites including the thalamus and hippocampus. Although PKC-delta has been implicated in regulating hypothalamic glucose homeostasis, its distribution in the hypothalamus has not previously been described. In the current study, we used immunohistochemistry to examine the distribution of PKC-theta and -delta immunoreactivity in rat and mouse hypothalamus. We found PKC-theta immunoreactive neurons in several hypothalamic nuclei including the ARC, lateral hypothalamic area, perifornical area and tuberomammillary nucleus. PKC-delta immunoreactive neurons were found in the paraventricular and supraoptic nuclei. Double-label immunohistochemisty in mice expressing green fluorescent protein either with the long form of leptin receptor (LepR-b) or in orexin (ORX) neurons indicated that PKC-theta is highly colocalized in lateral hypothalamic ORX neurons but not in lateral hypothalamic LepR-b neurons. Double-label immunohistochemistry in oxytocin-enhanced yellow fluorescent protein mice or arginine vasopressin-enhanced green fluorescent protein (AVP-EGFP) transgenic rats revealed a high degree of colocalization of PKC-delta within paraventricular and supraoptic oxytocin neurons but not the vasopressinergic neurons. We conclude that PKC-theta and -delta are expressed in different hypothalamic neuronal populations. |
