| First Author | Miller MR | Year | 2017 |
| Journal | J Cell Sci | Volume | 130 |
| Issue | 10 | Pages | 1730-1739 |
| PubMed ID | 28348104 | Mgi Jnum | J:250144 |
| Mgi Id | MGI:5924481 | Doi | 10.1242/jcs.195099 |
| Citation | Miller MR, et al. (2017) Non-canonical activity of the podosomal formin FMNL1gamma supports immune cell migration. J Cell Sci 130(10):1730-1739 |
| abstractText | Having previously located the formin FMNL1 in macrophage podosomes, we developed an in vivo model to assess the role of FMNL1 in the migration activities of primary macrophages. Deletion of FMNL1 in mice was genetically lethal; however, targeted deletion in macrophages was achieved by employing macrophage-specific Cre. Unchallenged FMNL1-deficient mice exhibited an unexpected reduction in tissue-resident macrophages despite normal blood monocyte numbers. Upon immune stimulus, the absence of FMNL1 resulted in reduced macrophage recruitment in vivo, decreased migration in two-dimensional in vitro culture and a decrease in the number of macrophages exhibiting podosomes. Of the three described isoforms of FMNL1 - alpha, beta and gamma - only FMNL1gamma rescued macrophage migration when expressed exogenously in depleted macrophages. Surprisingly, mutation of residues in the FH2 domain of FMNL1gamma that disrupt barbed-end actin binding did not limit rescue of macrophage migration and podosome numbers. These observations suggest that FMNL1 contributes to macrophage migration activity by stabilizing the lifespan of podosomes without interaction of fast-growing actin termini. |
