| First Author | Lee B | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 6 | Pages | e99575 |
| PubMed ID | 24911652 | Mgi Jnum | J:217326 |
| Mgi Id | MGI:5613757 | Doi | 10.1371/journal.pone.0099575 |
| Citation | Lee B, et al. (2014) Macrophage depletion disrupts immune balance and energy homeostasis. PLoS One 9(6):e99575 |
| abstractText | Increased macrophage infiltration in tissues including white adipose tissue and skeletal muscle has been recognized as a pro-inflammatory factor that impairs insulin sensitivity in obesity. However, the relationship between tissue macrophages and energy metabolism under non-obese physiological conditions is not clear. To study a homeostatic role of macrophages in energy homeostasis, we depleted tissue macrophages in adult mice through conditional expression of diphtheria toxin (DT) receptor and DT-induced apoptosis. Macrophage depletion robustly reduced body fat mass due to reduced energy intake. These phenotypes were reversed after macrophage recovery. As a potential mechanism, severe hypothalamic and systemic inflammation was induced by neutrophil (NE) infiltration in the absence of macrophages. In addition, macrophage depletion dramatically increased circulating granulocyte colony-stimulating factor (G-CSF) which is indispensable for NE production and tissue infiltration. Our in vitro study further revealed that macrophages directly suppress G-CSF gene expression. Therefore, our study indicates that macrophages may play a critical role in integrating immune balance and energy homeostasis under physiological conditions. |
