| First Author | Riding AM | Year | 2022 |
| Journal | iScience | Volume | 25 |
| Issue | 7 | Pages | 104660 |
| PubMed ID | 35845169 | Mgi Jnum | J:328687 |
| Mgi Id | MGI:7316124 | Doi | 10.1016/j.isci.2022.104660 |
| Citation | Riding AM, et al. (2022) Group 3 innate lymphocytes make a distinct contribution to type 17 immunity in bladder defence. iScience 25(7):104660 |
| abstractText | Bladder infection affects a hundred million people annually, but our understanding of bladder immunity is incomplete. We found type 17 immune response genes among the most up-regulated networks in mouse bladder following uropathogenic Escherichia coli (UPEC) challenge. Intravital imaging revealed submucosal Rorc+ cells responsive to UPEC challenge, and we found increased Il17 and IL22 transcripts in wild-type and Rag2 (-/-) mice, implicating group 3 innate lymphoid cells (ILC3s) as a source of these cytokines. NCR-positive and negative ILC3 subsets were identified in murine and human bladders, with local proliferation increasing IL17-producing ILC3s post infection. ILC3s made a more limited contribution to bladder IL22, with prominent early induction of IL22 evident in Th17 cells. Single-cell RNA sequencing revealed bladder NCR-negative ILC3s as the source of IL17 and identified putative ILC3-myeloid cell interactions, including via lymphotoxin-beta-LTBR. Altogether, our data provide important insights into the orchestration and execution of type 17 immunity in bladder defense. |
