| First Author | Honey K | Year | 2002 |
| Journal | Nat Immunol | Volume | 3 |
| Issue | 11 | Pages | 1069-74 |
| PubMed ID | 12368909 | Mgi Jnum | J:109255 |
| Mgi Id | MGI:3626168 | Doi | 10.1038/ni844 |
| Citation | Honey K, et al. (2002) Thymocyte expression of cathepsin L is essential for NKT cell development. Nat Immunol 3(11):1069-74 |
| abstractText | CD1d antigen presentation to natural killer T (NKT) cells expressing the semi-invariant T cell receptor V(alpha)14J(alpha)18 requires CD1d trafficking through endosomal compartments; however, the endosomal events remain undefined. We show that mice lacking the endosomal protease cathepsin L (catL) have greatly reduced numbers of V(alpha)14(+)NK1.1(+) T cells. In addition, catL expression in thymocytes is critical not only for selection of these cells in vivo but also for stimulation of V(alpha)14(+)NK1.1(+) T cells in vitro. CD1d cell-surface expression and intracellular localization appear normal in catL-deficient thymocytes, as does the lysosomal morphology; this implies a specific role for catL in regulating presentation of natural CD1d ligands mediating V(alpha)14(+)NK1.1(+) T cell selection. These data implicate lysosomal proteases as key regulators of not only classical major histocompatibility complex class II antigen presentation but also nonclassical CD1d presentation. |
