| First Author | Rube HT | Year | 2016 |
| Journal | Nat Commun | Volume | 7 |
| Pages | 11025 | PubMed ID | 27008915 |
| Mgi Jnum | J:236741 | Mgi Id | MGI:5807011 |
| Doi | 10.1038/ncomms11025 | Citation | Rube HT, et al. (2016) Sequence features accurately predict genome-wide MeCP2 binding in vivo. Nat Commun 7:11025 |
| abstractText | Methyl-CpG binding protein 2 (MeCP2) is critical for proper brain development and expressed at near-histone levels in neurons, but the mechanism of its genomic localization remains poorly understood. Using high-resolution MeCP2-binding data, we show that DNA sequence features alone can predict binding with 88% accuracy. Integrating MeCP2 binding and DNA methylation in a probabilistic graphical model, we demonstrate that previously reported genome-wide association with methylation is in part due to MeCP2's affinity to GC-rich chromatin, a result replicated using published data. Furthermore, MeCP2 co-localizes with nucleosomes. Finally, MeCP2 binding downstream of promoters correlates with increased expression in Mecp2-deficient neurons. |
