| First Author | Wang Y | Year | 2012 |
| Journal | Genesis | Volume | 50 |
| Issue | 7 | Pages | 534-42 |
| PubMed ID | 22139977 | Mgi Jnum | J:187502 |
| Mgi Id | MGI:5437204 | Doi | 10.1002/dvg.20832 |
| Citation | Wang Y, et al. (2012) Generation of mice with conditional null allele for GdX/Ubl4A. Genesis 50(7):534-42 |
| abstractText | GdX (also named Ubl4A) is a house-keeping gene located on the X chromosome and encodes a protein harboring an ubiquitin-like domain in human and mouse. Although identified in 1988, the function of GdX remains unknown. To elucidate the role of GdX in vivo, we generated a conditional GdX knockout mouse in which Exon 2 was flanked by two loxP sites. We obtained viable and fertile mice with homozygous GdX(flox/flox) or GdX(flox/Y) allele. Germ-line transmission was confirmed by crossing the mouse bearing conditionally targeted allele with an EIIalpha-Cre transgenic mouse. GdX was successfully depleted in tissues of EIIalpha-Cre-GdX-null mice. GdX(-/-) and GdX(-/Y) mice are viable and exhibit normal development compared with wild-type littermates within 6 months during our observation. We also observed that GdX knockout male mice were functionally normal in the reproductive system where Ubl4B was specifically expressed. GdX(flox/flox) and GdX(flox/Y) conditional mice provide a tool for further tissue-specific function analysis of the GdX protein under different conditions. |
