| First Author | Klatt O | Year | 2021 |
| Journal | Cell Rep | Volume | 35 |
| Issue | 11 | Pages | 109266 |
| PubMed ID | 34133920 | Mgi Jnum | J:314763 |
| Mgi Id | MGI:6717067 | Doi | 10.1016/j.celrep.2021.109266 |
| Citation | Klatt O, et al. (2021) Endogenous beta-neurexins on axons and within synapses show regulated dynamic behavior. Cell Rep 35(11):109266 |
| abstractText | Neurexins are key organizer molecules that regulate synaptic function and are implicated in autism and schizophrenia. beta-neurexins interact with numerous cell adhesion and receptor molecules, but their neuronal localization remains elusive. Using single-molecule tracking and high-resolution microscopy to detect neurexin1beta and neurexin3beta in primary hippocampal neurons from knockin mice, we demonstrate that endogenous beta-neurexins are present in fewer than half of excitatory and inhibitory synapses. Moreover, we observe a large extrasynaptic pool of beta-neurexins on axons and show that axonal beta-neurexins diffuse with higher surface mobility than those transiently confined within synapses. Stimulation of neuronal activity further increases the mobility of synaptic and axonal beta-neurexins, whereas inhibition causes the opposite. Blocking ectodomain cleavage by metalloproteases also reduces beta-neurexin mobility and enhances glutamate release. These findings suggest that the surface mobility of endogenous beta-neurexins inside and outside of synapses is dynamically regulated and linked to neuronal activity. |
