| First Author | Sasman A | Year | 2012 |
| Journal | Genesis | Volume | 50 |
| Issue | 10 | Pages | 766-74 |
| PubMed ID | 22522965 | Mgi Jnum | J:188816 |
| Mgi Id | MGI:5442261 | Doi | 10.1002/dvg.22036 |
| Citation | Sasman A, et al. (2012) Generation of conditional alleles for Foxc1 and Foxc2 in mice. Genesis 50(10):766-74 |
| abstractText | The Forkhead box transcription factors, Foxc1 and Foxc2, are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell-type specific and postdevelopmental functions are unknown, in part because conventional (i.e., whole-organism) homozygous-null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional-null Foxc1(flox) and Foxc2(flox) mutations that are induced via Cre-mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild-type function. The embryos of Foxc1(flox) or Foxc2(flox) mice crossed with Cre-deleter mice that are homozygous for the recombined allele (i.e., Foxc1(Delta/Delta) or Foxc2(Delta/Delta) embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell-type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals. genesis 50:766-774, 2012. (c) 2012 Wiley Periodicals, Inc. |
