| First Author | Farrelly O | Year | 2021 |
| Journal | Cell Stem Cell | Volume | 28 |
| Issue | 7 | Pages | 1233-1247.e4 |
| PubMed ID | 33984283 | Mgi Jnum | J:347541 |
| Mgi Id | MGI:6874781 | Doi | 10.1016/j.stem.2021.02.022 |
| Citation | Farrelly O, et al. (2021) Two-photon live imaging of single corneal stem cells reveals compartmentalized organization of the limbal niche. Cell Stem Cell 28(7):1233-1247.e4 |
| abstractText | The functional heterogeneity of resident stem cells that support adult organs is incompletely understood. Here, we directly visualize the corneal limbus in the eyes of live mice and identify discrete stem cell niche compartments. By recording the life cycle of individual stem cells and their progeny, we directly analyze their fates and show that their location within the tissue can predict their differentiation status. Stem cells in the inner limbus undergo mostly symmetric divisions and are required to sustain the population of transient progenitors that support corneal homeostasis. Using in situ photolabeling, we captured their progeny exiting the niche before moving centripetally in unison. The long-implicated slow-cycling stem cells are functionally distinct and display local clonal dynamics during homeostasis but can contribute to corneal regeneration after injury. This study demonstrates how the compartmentalized organization of functionally diverse stem cell populations supports the maintenance and regeneration of an adult organ. |
