| First Author | Rhee JS | Year | 2002 |
| Journal | Cell | Volume | 108 |
| Issue | 1 | Pages | 121-33 |
| PubMed ID | 11792326 | Mgi Jnum | J:74347 |
| Mgi Id | MGI:2158119 | Doi | 10.1016/s0092-8674(01)00635-3 |
| Citation | Rhee JS, et al. (2002) Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs. Cell 108(1):121-33 |
| abstractText | Munc13-1 is a presynaptic protein with an essential role in synaptic vesicle priming. It contains a diacylglycerol (DAG)/beta phorbol ester binding C(1) domain and is a potential target of the DAG second messenger pathway that may act in parallel with PKCs. Using genetically modified mice that express a DAG/beta phorbol ester binding-deficient Munc13-1(H567K) variant instead of the wild-type protein, we determined the relative contribution of PKCs and Munc13-1 to DAG/beta phorbol ester-dependent regulation of neurotransmitter release. We show that Munc13s are the main presynaptic DAG/beta phorbol ester receptors in hippocampal neurons. Modulation of Munc13-1 activity by second messengers via the DAG/beta phorbol ester binding C(1) domain is essential for use-dependent alterations of synaptic efficacy and survival. |
