| Primary Identifier | MGI:2148771 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Calca |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Two separate functional peptides, calcitonin and calcitonin gene related peptide-alpha (CGRP-alpha), are derived from this locus by alternative splicing. Calcitonin is encoded by exon 4 and CGRP-alpha by exon 5. Part of exon 5 was replaced with a lacZ gene and neomycin selection gene. Northern blot and RT-PCR analysis on samples derived from spinal root ganglia of homozygous mice demonstrated that no detectable CGRP-alpha transcript was produced from this allele. Western blot analysis on spinal ganglia extracts derived from homozygous mice confirmed that no detectable CGRP-alpha was made. Precursor calcitonin mRNA was detected in the spinal cord. |
