| First Author | Corey EA | Year | 2021 |
| Journal | Mol Cell Neurosci | Volume | 110 |
| Pages | 103585 | PubMed ID | 33358996 |
| Mgi Jnum | J:313797 | Mgi Id | MGI:6707730 |
| Doi | 10.1016/j.mcn.2020.103585 | Citation | Corey EA, et al. (2021) Inhibitory signaling in mammalian olfactory transduction potentially mediated by Galphao. Mol Cell Neurosci 110:103585 |
| abstractText | Olfactory GPCRs (ORs) in mammalian olfactory receptor neurons (ORNs) mediate excitation through the Galphas family member Galphaolf. Here we tentatively associate a second G protein, Galphao, with inhibitory signaling in mammalian olfactory transduction by first showing that odor evoked phosphoinositide 3-kinase (PI3K)-dependent inhibition of signal transduction is absent in the native ORNs of mice carrying a conditional OMP-Cre based knockout of Galphao. We then identify an OR from native rat ORNs that are activated by octanol through cyclic nucleotide signaling and inhibited by citral in a PI3K-dependent manner. We show that the OR activates cyclic nucleotide signaling and PI3K signaling in a manner that reflects its functionality in native ORNs. Our findings lay the groundwork to explore the interesting possibility that ORs can interact with two different G proteins in a functionally identified, ligand-dependent manner to mediate opponent signaling in mature mammalian ORNs. |
