| First Author | Chagin AS | Year | 2014 |
| Journal | Nat Commun | Volume | 5 |
| Pages | 3673 | PubMed ID | 24781502 |
| Mgi Jnum | J:213233 | Mgi Id | MGI:5583924 |
| Doi | 10.1038/ncomms4673 | Citation | Chagin AS, et al. (2014) G-protein stimulatory subunit alpha and Gq/11alpha G-proteins are both required to maintain quiescent stem-like chondrocytes. Nat Commun 5:3673 |
| abstractText | Round chondrocytes in the resting zone of the growth plate provide precursors for columnar chondrocytes and have stem-like properties. Here we demonstrate that these stem-like chondrocytes undergo apoptosis in the absence of the receptor (PPR) for parathyroid hormone-related protein. We examine the possible roles of heterotrimeric G-proteins activated by the PPR. Inactivation of the G-protein stimulatory alpha-subunit (G(s)alpha) leads to accelerated differentiation of columnar chondrocytes, as seen in the PPR knockout, but a remnant of growth cartilage remains, in contrast to disappearance of the growth cartilage in the PPR knockout. Stem-like chondrocytes lose their quiescence and proliferate upon G(s)alpha ablation. Inactivation of G(s)alpha in mice with a mutant PPR that cannot activate G proteins, Gq and G11, leads to a PPR knockout-like phenotype. Thus, G(s)alpha is the major mediator of the anti-differentiation action of the PPR, while activation of both G(s)alpha and Gq/11alpha is required for quiescence of stem-like chondrocytes. |
