| First Author | Harada A | Year | 2002 |
| Journal | J Cell Biol | Volume | 158 |
| Issue | 3 | Pages | 541-9 |
| PubMed ID | 12163474 | Mgi Jnum | J:78263 |
| Mgi Id | MGI:2183872 | Doi | 10.1083/jcb.200110134 |
| Citation | Harada A, et al. (2002) MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction. J Cell Biol 158(3):541-9 |
| abstractText | Microtubule-associated protein 2 (MAP2) is a major component of cross-bridges between microtubules in dendrites, and is known to stabilize microtubules. MAP2 also has a binding domain for the regulatory subunit II of cAMP-dependent protein kinase (PKA). We found that there is reduction in microtubule density in dendrites and a reduction of dendritic length in MAP2-deficient mice. Moreover, there is a significant reduction of various subunits of PKA in dendrites and total amounts of various PKA subunits in hippocampal tissue and cultured neurons. In MAP2-deficient cultured neurons, the induction rate of phosphorylated CREB after forskolin stimulation was much lower than in wild-type neurons. Therefore, MAP2 is an anchoring protein of PKA in dendrites, whose loss leads to reduced amount of dendritic and total PKA and reduced activation of CREB. |
