| Primary Identifier | MGI:2152589 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Pkd1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A promoterless IRES-LacZ-neomycin resistance cassette containing a 5' En-2 intron and splice acceptor site replaced exons 17-21. Northern analysis of homozygous mutant E12.5 embryos using an exon 15 probe detected a 12.5kb fusion transcript, and did not detect the wild-type transcript. Western analysis using an anti-beta-galactosidase antibody detected the predicted 146kDa beta-galactosidase-neomycin fusion protein in homozygous mutant embryos. |
