| First Author | Rhieu BH | Year | 2014 |
| Journal | In Vivo | Volume | 28 |
| Issue | 4 | Pages | 449-56 |
| PubMed ID | 24982209 | Mgi Jnum | J:333581 |
| Mgi Id | MGI:6882435 | Citation | Rhieu BH, et al. (2014) Increased hematopoiesis in long-term bone marrow cultures and reduced irradiation-induced pulmonary fibrosis in Von Willebrand factor homologous deletion recombinant mice. In Vivo 28(4):449-56 |
| abstractText | AIM: We investigated whether homologous recombinant deletion of the endothelial cell-specific protein Von Willebrand factor (vWF) affected hematopoiesis in long-term bone marrow cultures, and irradiation induction of pulmonary fibrosis/organizing alveolitis. MATERIALS AND METHODS: We established long-term bone marrow cultures from vWF(-/-) (C57BL/6 background) and vWF(+/+) littermate mice. Non-adherent cells removed weekly were tested for formation of multi-lineage hematopoietic stem cells forming colonies at 7 and 14 days in secondary semi-solid medium cultures. Irradiation fibrosis in the lungs of 20-Gy thoracic irradiated mice was quantitated and scored. RESULTS: Hematopoiesis was increased over 20 weeks in vWF(-/-) compared to vWF(+/+) cultures in production of non-adherent cells, and cells forming colonies at 7 or 14 days in secondary semi-solid medium culture. The irradiated lungs showed no increased fibrosis. CONCLUSION: Absence of vWF increases hematopoiesis in long-term bone marrow cultures and has a protective effect in irradiated lungs. |
