| First Author | David A | Year | 2014 |
| Journal | Cell Cycle | Volume | 13 |
| Issue | 18 | Pages | 2859-68 |
| PubMed ID | 25486474 | Mgi Jnum | J:316794 |
| Mgi Id | MGI:6840592 | Doi | 10.4161/15384101.2014.946830 |
| Citation | David A, et al. (2014) Lack of centrioles and primary cilia in STIL(-/-) mouse embryos. Cell Cycle 13(18):2859-68 |
| abstractText | Although most animal cells contain centrosomes, consisting of a pair of centrioles, their precise contribution to cell division and embryonic development is unclear. Genetic ablation of STIL, an essential component of the centriole replication machinery in mammalian cells, causes embryonic lethality in mice around mid gestation associated with defective Hedgehog signaling. Here, we describe, by focused ion beam scanning electron microscopy, that STIL(-/-) mouse embryos do not contain centrioles or primary cilia, suggesting that these organelles are not essential for mammalian development until mid gestation. We further show that the lack of primary cilia explains the absence of Hedgehog signaling in STIL(-/-) cells. Exogenous re-expression of STIL or STIL microcephaly mutants compatible with human survival, induced non-templated, de novo generation of centrioles in STIL(-/-) cells. Thus, while the abscence of centrioles is compatible with mammalian gastrulation, lack of centrioles and primary cilia impairs Hedgehog signaling and further embryonic development. |
