| Primary Identifier | MGI:2153000 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Col10a1 |
| Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance cassette was inserted into exon 3, disrupting sequences encoding the beginning of the helix domain. RT-PCR assays did not detect expression of a normal transcript in cartilage RNA derived from homozygous mice, although low levels of a mutant transcript were detected. Immunohistochemical analysis on cartilage of 2 day old homozygous mice confirmed the deficiency of the protein. |
